Regulation of Cytosolic Phospholipase A2 Phosphorylation by Proteolytic Cleavage of Annexin A1 in Activated Mast Cells
نویسندگان
چکیده
منابع مشابه
Regulation of cytosolic phospholipase A2 by phosphorylation.
Introduction Phospholipases catalyse the cleavage of membrane phospholipids and thereby generate second messengers that participate in intracellular signal transduction processes or act as precursors of tissue hormones. Cytosolic phospholipase Az (cPLA2) is an 85 kDa enzyme that cleaves arachidonic acid at the sn-2 position of the phospholipid [1,2]. The intracellular location of the enzyme ena...
متن کاملInhibition of human cytosolic phospholipase A2 by human annexin V.
The ability of annexins, particularly annexin 1 (lipocortin 1), to inhibit phospholipase A2 (PLA2) is well known and a substrate depletion mechanism is now widely accepted as the explanation for most inhibitory studies. However, there are only a very limited number of reported studies involving annexins and the high-molecular-mass cytosolic PLA2 (cPLA2). In this study we have examined the effec...
متن کاملSecretory phospholipase A2 receptor-mediated activation of cytosolic phospholipase A2 in murine bone marrow-derived mast cells.
The current study examined the signal transduction steps involved in the selective release of arachidonic acid (AA) induced by the addition of secretory phospholipase A2 (sPLA2) isotypes to bone marrow-derived mast cells (BMMC). Overexpression of sPLA2 receptors caused a marked increase in AA and PGD2 release after stimulation of BMMC, implicating sPLA2 receptors in this process. The hypothesis...
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Cytosolic phospholipase A2 (cPLA2) mediates agonist-induced arachidonic acid release, the first step in eicosanoid production. cPLA2 is regulated by phosphorylation and by calcium, which binds to a C2 domain and induces its translocation to membrane. The functional roles of phosphorylation sites and the C2 domain of cPLA2 were investigated. In Sf9 insect cells expressing cPLA2, okadaic acid, an...
متن کاملRegulation of arachidonic acid release and cytosolic phospholipase A2 activation.
The 85-kDa cytosolic PLA2 (cPLA2) mediates agonist-induced arachidonic acid release in many cell models, including mouse peritoneal macrophages. cPLA2 is regulated by an increase in intracellular calcium, which binds to an amino-terminal C2 domain and induces its translocation to the nuclear envelope and endoplasmic reticulum. Phosphorylation of cPLA2 on S505 by mitogen-activated protein kinase...
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ژورنال
عنوان ژورنال: The Journal of Immunology
سال: 2012
ISSN: 0022-1767,1550-6606
DOI: 10.4049/jimmunol.1102306